Laboratory for Metabolomics

 

 

 

   Lipids are recognized as extremely diverse molecules. Precise determination of molecular lipid species becomes a prerequisite not only to understand their biological functions in physiology and disease, but also to discover novel bioactive lipids that may link between lipid metabolisms and biological phenotypes. A powerful method for the analysis of lipids is liquid chromatography tandem mass spectrometry (LC-MS/MS). 

 

   Our research is aimed at elucidating structure and function of endogenous lipid mediators that regulate inflammation and tissue homeostasis. Polyunsaturated fatty acid (PUFA)-derived mediators are formed by enzymatic oxidation through the action of cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 monooxygenases (CYP).  By using LC-MS/MS-based lipidomics, we provided new insights into the cellular and molecular mechanisms of inflammatory resolution, especially the functional roles of 12/15-LOX-expressing eosinophils and macrophages in controlling the resolution of inflammation. 

 

   Also our research is focused on understanding omega-3 PUFA’s function using genetic and lipidomic approaches, and has identified novel metabolic pathway and bioactive metabolites that may link to omega-3 PUFA’s anti-inflammatory actions. These endogenous mediators with potent anti-inflammatory property could lead to the development of novel therapeutics for disease when sustained inflammation is suspected as key components of pathogenesis.

 

   Also we put a lot of efforts into development and application of target and non-target multi-lipidomics platform to discover novel link between lipid metabolism and biological phenotypes. 

 

 

 


脂肪酸代謝バランスと脂質の三大機能

     実験医学(羊土社)2012年2月号より引用

 


 

Links

[For IMS Center] Information on metabolome analysis support

 

Science Webinar (6 May 2020)

Linking lipids and disease: Making the connection between fatty acids, inflammation, and tissue homeostasis

 

LipoQuality -Quality of Lipids in Biological Systems-

 

60th International Conference on the Bioscience of Lipids WebSite

 

Lipidomics software tools(RIKEN PRIMe)

 

 

Keio University Faculty of Pharmacy   Division of Physiological Chemistry and Metabolism

 

Yokohama City University   Cellular and Molecular Epigenetics Laboratory