Laboratory for Metabolomics
Lipids are recognized as extremely diverse molecules. Precise determination of molecular lipid species becomes a prerequisite not only to understand their biological functions in physiology and disease, but also to discover novel bioactive lipids that may link between lipid metabolisms and biological phenotypes. A powerful method for the analysis of lipids is liquid chromatography tandem mass spectrometry (LC-MS/MS).
Our research is aimed at elucidating structure and function of endogenous lipid mediators that regulate inflammation and tissue homeostasis. Polyunsaturated fatty acid (PUFA)-derived mediators are formed by enzymatic oxidation through the action of cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 monooxygenases (CYP). By using LC-MS/MS-based lipidomics, we provided new insights into the cellular and molecular mechanisms of inflammatory resolution, especially the functional roles of 12/15-LOX-expressing eosinophils and macrophages in controlling the resolution of inflammation.
Also our research is focused on understanding omega-3 PUFA’s function using genetic and lipidomic approaches, and has identified novel metabolic pathway and bioactive metabolites that may link to omega-3 PUFA’s anti-inflammatory actions. These endogenous mediators with potent anti-inflammatory property could lead to the development of novel therapeutics for disease when sustained inflammation is suspected as key components of pathogenesis.
Also we put a lot of efforts into development and application of target and non-target multi-lipidomics platform to discover novel link between lipid metabolism and biological phenotypes.
|[For IMS Center]||Information on metabolome analysis support|
|Keio University||Faculty of Pharmacy|
|Yokohama City University||Cellular and Molecular Epigenetics Laboratory|