Laboratory for Metabolomics

   Lipids are recognized as extremely diverse molecules. Precise determination of molecular lipid species becomes a prerequisite not only to understand their biological functions in physiology and disease, but also to discover novel bioactive lipids that may link between lipid metabolisms and biological phenotypes. A powerful method for the analysis of lipids is liquid chromatography tandem mass spectrometry (LC-MS/MS). 

 

   Our research is aimed at elucidating structure and function of endogenous lipid mediators that regulate inflammation and tissue homeostasis. Polyunsaturated fatty acid (PUFA)-derived mediators are formed by enzymatic oxidation through the action of cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 monooxygenases (CYP).  By using LC-MS/MS-based lipidomics, we provided new insights into the cellular and molecular mechanisms of inflammatory resolution, especially the functional roles of 12/15-LOX-expressing eosinophils and macrophages in controlling the resolution of inflammation. 

 

   Also our research is focused on understanding omega-3 PUFA’s function using genetic and lipidomic approaches, and has identified novel metabolic pathway and bioactive metabolites that may link to omega-3 PUFA’s anti-inflammatory actions. These endogenous mediators with potent anti-inflammatory property could lead to the development of novel therapeutics for disease when sustained inflammation is suspected as key components of pathogenesis.

 

   Also we put a lot of efforts into development and application of target and non-target multi-lipidomics platform to discover novel link between lipid metabolism and biological phenotypes. 

 

 

 


脂肪酸代謝バランスと脂質の三大機能

      実験医学(羊土社)2012年2月号より引用

 


Links

LipoQuality -Quality of Lipids in Biological Systems-

 

60th International Conference on the Bioscience of Lipids WebSite

 

Keio University Faculty of Pharmacy   Division of Physiological Chemistry and Metabolism

 

Yokohama City University   Cellular and Molecular Epigenetics Laboratory