Lipids are extremely diverse molecules, but their diversity, structure, and 46Figure: Untargeted lipidomics to reveal the con-nection between lipid metabolism and tissue homeostasisexact function are not well understood. Precise determination of each molecular species of lipid is a prerequisite for understanding their functions in physiology and disease, and for discovering novel, bioactive lipid-derived mediators that may have therapeutic benefits. Liquid chromatography tandem mass spectrometry (LC-MS/MS) is a powerful method for analyzing lipid me-tabolites, providing insight into the structure and function of endogenous lipid metabolites that regulate inflammation and tissue homeostasis.We established an untargeted lipidomics platform packaged in MS-DIAL4 that derived a catalog of over 8,000 unique structures of 117 lipid class catego-ries. We created a comprehensive database of lipid structures that includes their mass spectrum properties such as retention time, collision cross section and mass fragmentation pattern to correctly characterize lipids, and this has been published as a “lipidome atlas”. This untargeted lipidomics platform is a power-ful technology for visualizing lipid networks globally. It also provides an oppor-tunity for data-driven hypothesis generation to make the connection between lipid metabolism and biological phenotypes. We applied this system to under-stand the gut microbiota-associated unique lipid structures and their complex metabolic networks in mice.Recent Major PublicationsYasuda S, Okahashi N, Tsugawa H, Ogata Y, Ikeda K, Suda W, Arai H, Hattori M, Arita M. Elucidation of gut microbiota-associated lipids using LC-MS/MS and 16S rRNA sequence analyses. iScience 23, 101841 (2020)Tsugawa H, Ikeda K, Takahashi M, Satoh A, Mori Y, Uchino H, Okahashi N, Yamada Y, Tada I, Bonini P, Higashi Y, Okazaki Y, Zhou Z, Zhu Z, Koelmel J, Cajka T, Fiehn O, Saito K, Arita M, Arita M. A lipidome atlas in MS-DIAL 4. Nat Biotechnol 38, 1159-1163 (2020)Ogawa M, Ishihara T, Isobe Y, Kato T, Kuba K, Imai Y, Uchino Y, Tsubota K, Arita M. Eosinophils promote corne-al wound healing via the 12/15-lipoxygenase pathway. FASEB J 34, 12492-12501 (2020)Invited presentationsArita M. “Biology of LipoQuality: Advanced lipidomics technology and its application in biology” The 43rd An-nual Meeting of the Molecular Biology Society of Japan (Online) December 2020Arita M. “Advanced lipidomics technology and its ap-plication in biology” The 45th Annual Meeting of the Japanese Society for Biomedical Mass Spectrometry (Online) September 2020Arita M. “Linking lipid metabolism and inflammatory disorders” The 93rd Annual Meeting of the Japanese Biochemical Society (Online) September 2020Arita M. “Biology of LipoQuality: Omega-3 fatty acid cascade that controls inflammation and tissue homeo-stasis” Science Webinar (Online) May 2020Laboratory for MetabolomicsTeam Leader: Makoto Arita
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