66Figure: LC-MS/MS-based Advanced Lipidomics PlatformFigure: The computer servers managed in the West building (left) and in the East building (right)as energy sources, signaling molecules, and their precursors. Some lipids are biologically active as single molecules, while oth-ers are involved in forming membrane domains as molecular as-semblies. In general, the dysregulation of lipid metabolism is as-sociated with human diseases and, hence, the precise determina-tion of lipids is quite important for understanding their functions in physiology and disease, and for discovering novel bioactive lipids that may have therapeutic benefits.Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful method for analyzing lipid metabolites. The Metabolomics Laboratory is equipped with an AQUITY UPLC (Waters) coupled with a TripleTOF 6600, a QTRAP 6500/5500/4500 (Sciex), and a GC-MS TQ8030 (Shimadzu) to cover untargeted and wide-targeted metabolomics. Recently, we introduced a timsTOF flex MALDI-2 (Bruker) for MS imaging (spatial lipidomics) analyses. This state-of-the-art lipidomics platform together with our original informatics technologies are powerful tools for elucidating complex lipid structures and visual-research activities in IMS, several groups manage the infor-mation infrastructure and computational resources. In the West building, we have internal large-scale computation systems (total ~1,000 cores, 3.3PB hot storage) using RIKEN’s internal network and provide them for IMS researchers and manage external web-sites for several large-scale collaboration projects in IMS. We have also utilized HOKUSAI SS to expand the in-house computation systems by providing a seamless environment. In the East build-ing, we also have internal large-scale computation systems (total ~3500 cores, 8PB storage) in a closed network and provide wide access for IMS researchers, not just those in the East building; other buildings also have access to these servers, especially for controlled access data, e.g., human-derived data. We are planning to gradually shift the storage system to HOKUSAI SR to securely store human genomics data. Moreover, two remote access termi-nals are available in the East building for IMS researchers to easily access Tohoku Medical Megabank (ToMMo) data.Recently, the sharing of research data has become important in the open science era. We have drafted IMS Research Data Guidelines for the treatment of research data obtained in IMS, for izing metabolic networks globally and in an unbiased and provide an opportunity for data-driven hypotheses to make the connec-tion between lipid metabolism and biological phenotypes.discussion in IMS.Large-scale computation is now essential in biomedical studies and we continue to provide sufficient and the latest computation environment and information infrastructure for IMS researchers.Information InfrastructureTo promote and support large-scale computation for various Metabolomics (Lipidomics)Lipids constitute biological membranes and play various roles
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