23715Table: Instruments and their usage in the FACS Laboratory (2023)Insturment typeFACS cell analyzerFACS cell sorterMass cytometer (CyTOF)Figure: Leica SP8 confocal microscope (1), Leica SP8 multiphoton micro-scope (2), Nikon N-SIM/N-STORM super-resolution microscope (4), GE Healthcare DeltaVision Elite system (5), Keyence BZ-X700 microscope (6), and Hitachi Regulus8420 FE-SEM (7)lowing services to users conducting research in various fields.(1) Technical support and training: Training courses (in Japanese and English) are held for each model (Table 1), and researchers who have completed the courses can use the model in question. Registered users can make reservations one month in advance via the in-house website.(1) Inverted Leica SP8 system equipped with hybrid detectors and (2) Inverted Leica SP8 system with two femtosecond Ti:Sa lasers for multiphoton excitation. This system is equipped with two types of scanners (resonant and galvano) and hybrid detectors. One of the two Ti:Sa lasers is connected to an optical paramet-ric oscillator (OPO) that enables two-photon imaging by long wavelength excitation.(3) Inverted Leica SP5 system with hybrid detectors.(4) Inverted Nikon N-SIM/N-STORM super-resolution microscope (5) GE Healthcare DeltaVision Elite system.(6) Keyence BZ-X700 all-in-one fluorescence microscope.(7) Hitachi field emission scanning electron microscope (FE-SEM) 64searchers in the Center with access to the most advanced equipment and technologies. The platforms consist of six sec-tions; the FACS managed by Dr. Jun Seita, the Imaging and Microscopes managed by Dr. Takaharu Okada, the Genome Plat-etry and cell sorting techniques essential to various IMS studies. In the FACS Laboratory, 5 analyzers and 7 cell sorters are in operation as instruments that use fluorescently labeled anti-bodies. This year, the Calibur generation of analyzers was discon-tinued, and the transition to Canto II and Celesta / Fortessa X-20 was completed. In addition, the Symphony generation cell sorter, which enables the simultaneous measurement of significantly higher dimensional parameters than ever before, is now in full operation. In addition, one CyTOF cytometer, HELIOS, which analyzes metal-labeled antibodies using the principle of mass spectrometry, is in operation.The professional staff of the FACS laboratory provides the fol-tissue imaging and coordinates technical support. There are six fluorescence microscopes and one scanning electron micro-scope available to researchers at IMS.the LIGHTNING super-resolution image extraction module.for dual color imaging.form and related activities managed by Drs. Yukihide Momozawa, Wataru Suda, Hiroshi Ohno, Jun Seita and Yasushi Okazaki, the Animal Facility managed by Dr. Haruhiko Koseki, Metabolomics (Lipidomics) managed by Dr. Makoto Arita, and Information In-frastructure managed by Dr. Takeya Kasukawa.(2) Cell sorting operation service: The FACS Laboratory offers a cell sorting operation service that allows users to request sorting experiments by experienced operators. In FY2023, we provided 54 such services.(3) Management and maintenance of FACS equipment: FACS equipment is kept ready for 24-hour operation through regular maintenance and technical support by professional staff and vendors.Regulus8240. The related devices such as a conventional ultra-microtome Leica EM UC7 and an array tomography ultrami-crotome ARTOS 3D are also available.Model# of machinesexperimentsCanto IICelesta/ Fortessa X-20Aria IIIu/III/ Fusion SymphonyHelios# of # of training sessions(participants)2 (15)5321153 (16)11244 (30)0 (0)IMS Research and Infrastructure PlatformsIMS Research and Infrastructure Platforms provide all re-FACSThe FACS Laboratory provides a wide range of flow cytom-Microscope LaboratoryThe Microscope Laboratory provides equipment for cell and
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