66Laboratory for Metabolomics, RIKEN-IMSLC-MS/MS-based Advanced Lipidomics PlatformFigure: LC-MS/MS-based Advanced Lipidomics Platformates complex biological systems, with lipids acting as key components of cellular membranes, as signaling molecules and as energy-storage molecules. In general, the dysregulation of lipid metabolism is associated with human diseases and, hence, the precise determination of lipids is quite important for understand-ing their functions in physiology and disease, and for discovering novel bioactive lipids that may have therapeutic benefits.A powerful method for analyzing lipid metabolites is liquid chromatography-tandem mass spectrometry (LC-MS/MS). The Metabolomics Laboratory is equipped with an AQUITY UPLC (Waters) coupled with a TripleTOF 6600, a QTRAP 6500/5500/4500 (Sciex) and a GC-MS TQ8030 (Shimadzu) to cover untargeted and wide-targeted metabolomics. Also, in 2022, we will introduce timsTOF flex MALDI-2 (Bruker) for MS imag-ing (spatial lipidomics) analyses. This state-of-the-art lipidomics platform together with our original informatics technologies are powerful tools for elucidating complex lipid structures and visual-izing metabolic networks globally and unbiasedly and provide an opportunity for data-driven hypotheses to make the connection between lipid metabolism and biological phenotypes.Metabolomics (Lipidomics)Lipids are extremely diverse molecules and such diversity cre-
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